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The
Daley laboratory has a central interest in the unique biology of pluripotent
cells, particularly mouse and human embryonic stem cells and germ cells.
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Our projects involve
work to determine genes and factors important for embryonic stem cell
self-renewal, directed differentiation technologies, somatic cell nuclear
transfer (SCNT), and transgenesis via retro and lentiviral targeting,
homologous recombination, and RNA interference strategies.
We also have a
keen interest in the specification of germ cells (both ova and sperm)
in early development and the establishment of methodologies to derive
such cells from an embryonic stem cell starting point.
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A
Wrights/Giemsa stained human embryonic stem cell (hESC) colony (H9) on
murine embryonic fibroblast (MEF) feeder cells at high magnification.
The colony contains roughly 50-70 individual hESC's.
photo: Willy Lensch
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GFP-positive
blastocysts developed by fertilizing murine oocytes with mouse ES cell derived
sperm. taken from: Geijsen N, Horoschak
M, Kim K, Gribnau J, Eggan K, Daley GQ. Derivation of embryonic germ cells
and male gametes from embryonic stem cells. Nature. 2004 Jan 8;427(6970):148-54.
(go
to abstract)
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H/E
stained teratoma sections derived in a NOD/SCID murine host from H9 hESC
after one month incubation time. The tumor demonstrates all three embryonic
germ layers (ectoderm, endoderm, and mesoderm) and is thus derivative of
a pluripotent cell. Clockwise from upper left: low
resolution view, skin epithelium (ectoderm), cartilage (mesoderm), brush
border respiratory epithelium (endoderm), muscinous gut epithelium (endoderm),
and smooth muscle (mesoderm). photo:
Willy Lensch |
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Day
7 hESC embryoid body in both phase contrast and fluorescent microscope images
following infection with an eGFP lentivirus. photo:
Willy Lensch |
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Return
to Daley Lab Homepage:  |
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