Yuin-Han Loh

Research Interests:

Pluripotency can be induced in mouse and human somatic cells by the forced expression of OCT4, SOX2 with either the combinations of KLF4, MYC or NANOG, LIN28 (Takahashi and Yamanaka, 2006; Takahashi et al., 2007; Yu et al., 2007; Park et al., 2007). In human, iPS cells are commonly generated from fibroblasts. However, the requirement to perform skin biopsy on the human donors and the need to grow the fibroblast cells for several passages in vitro has limited its use as an efficient source for generating patient-specific stem cells. We are directing our efforts to investigate novel ways of establishing iPS cells from cell types that are more accessible and requiring lesser manipulations. The ability to reprogram from these cells will facilitate the development of therapeutically safe and reliable methods towards the generation of patient-specific stem cells.



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			Yuin-Han Loh, Ph.D. Molecular Biology National University of Singapore - 2008 Post-Doctoral Fellow
			Research Interests: Pluripotency can be induced in mouse and human somatic cells by the forced expression of OCT4, SOX2 with either the combinations of KLF4, MYC or NANOG, LIN28 (Takahashi and Yamanaka, 2006; Takahashi et al., 2007; Yu et al., 2007; Park et al., 2007). In human, iPS cells are commonly generated from fibroblasts. However, the requirement to perform skin biopsy on the human donors and the need to grow the fibroblast cells for several passages in vitro has limited its use as an efficient source for generating patient-specific stem cells. We are directing our efforts to investigate novel ways of establishing iPS cells from cell types that are more accessible and requiring lesser manipulations. The ability to reprogram from these cells will facilitate the development of therapeutically safe and reliable methods towards the generation of patient-specific stem cells.
Articles in PubMed

	Selected Publications: 1) Loh, Y.H., Wu, Q., Chew, J.L., Vega, V.B., Zhang, W., Chen, X., Bourque, G., George, J., Leong, B., Liu, J., Wong, K.Y., Sung, K.W., Lee, C.W., Zhao, X.D., Chiu, K.P., Lipovich, L., Kuznetsov, V.A., Robson, P., Stanton, L.W., Wei, C.L., Ruan, Y., Lim, B., and Ng, H.H. (2006). The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics 38, 431-440. 2) Loh, Y.H., Zhang, W., Chen, X., George, J., Ng, H.H. (2007). Jmjd1a and Jmjd2c histone H3 lysine 9 demethylases regulate self-renewal in embryonic stem cells. Genes and Development 21, 2545-2557. 3) Jiang, J., Chan, Y.S, Loh, Y.H., Cai, J., Lim, C.A. Tong, G.Q., Zhong, S., and Ng, H.H. (2008). A core Klf circuitry that regulates self-renewal of embryonic stem cells. Nature Cell Biology 10, 353-360. Epub 2008 Feb 10. 4) Chen, X. Xu, H., Yuan, P., Fang, F., Huss, M., Vega, V., Wong, E., Orlov, Y., Zhang, W., Jiang, J., Loh, Y.H., Yeo, H.C., Yeo, Z.H., Narang, V., Govindarajan, K.R., Leong, B., Shahab, A., Ruan, Y., Bourque, G., Sung, W.K., Clarke, N., Wei, C.L., Ng, H.H. (2008) Integration of external signaling pathways with the core transcriptional network in embryonic stem cells. Cell 133(6):1106-1117. 5) Feng, B, Jiang, J., Kraus, P., Ng, J.H., Heng, J.C.D, Chan,Y.S., Yaw,L.P., Zhang,W. Loh, Y.H., Han, J., Vega, V.B., Cacheux-Rataboul,V., Lim, B., Lufkin, T., Ng, H.H. (2008) Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb. Nature Cell Biology (In press)